Anti-ALB/Albumin Antibody (AL-01) recommended for detection of ALB of human origin by WB and IP

Anti-ALB/Albumin Antibody (AL-01): sc-51515

Anti-ALB/Albumin Antibody (AL-01) is rated 4.0 out of 5 by 2.
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Datasheets

    • ALB/Albumin Antibody (AL-01) is a mouse monoclonal IgG1, cited in 10 publications, provided at 100 µg/ml
    • raised against fraction of proteins containing albumin purified from human serum
    • recommended for detection of ALB of human origin by WB and IP
    • See ALB/Albumin (F-10): sc-271605 for ALB/Albumin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ALB/Albumin Antibody (AL-01). This work is in progress.
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I am using ALB (AL-01): sc-51515 antibody for immunoprecipitation, how much antibody should I use?

Asked by: TinTin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Robust band seen in WB using Hep G2 whole Robust band seen in WB using Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-02-15
Rated 4 out of 5 by from Publishable data in WB using human suture Publishable data in WB using human suture mesenchymal cells. -SCBT Publication Review
Date published: 2013-03-17
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