Anti-Akt1 Antibody (A-11) recommended for detection of Akt1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Akt1 Antibody (A-11): sc-377457

Anti-Akt1 Antibody (A-11) is rated 4.6 out of 5 by 10.
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Datasheets

    • Akt1 Antibody (A-11) is a mouse monoclonal IgG1 κ, cited in 32 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 443-479 near the C-terminus of Akt1 of human origin
    • recommended for detection of Akt1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See Akt1 (B-1): sc-5298 for Akt1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Akt1 Antibody (A-11) for WB and IHC(P) applications. These reagents are now offered in bundles with Akt1 Antibody (A-11) (see ordering information below).
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What application is the blocking peptide sc-377457 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibodies I used this antibodies in dilution 1:1000. It works great.
Date published: 2018-06-26
Rated 4 out of 5 by from Great product The antibodies work in dilution 1:1000 (WB). It works good.
Date published: 2018-06-10
Rated 5 out of 5 by from antibody works well A nice choice for Akt1 detection and the concentration I used is 1:500.
Date published: 2017-06-01
Rated 5 out of 5 by from Very good Akt1 antibodies (11) : sc - 377457 provides beneficial data for experiment, which laid a foundation for future results, very good.
Date published: 2017-02-14
Rated 5 out of 5 by from Perfect It is a very good antibody, very good at every time, and there are very clear bands.
Date published: 2017-02-10
Rated 5 out of 5 by from Great for IHC(P) The Akt1 Antibody (A-11): sc-377457 provides excellent data for IHC with formalin fixed, paraffin-embedded tissue sections showing clean staining pattern. This antibody has also been cited multiple times for further verification.
Date published: 2017-01-24
Rated 5 out of 5 by from best antibody it is very best antibody , works great every time, good clear band with no background.Thanks
Date published: 2016-12-06
Rated 5 out of 5 by from Best The best Akt1 antibody , works great every time, good clear band with no background, try it you will never regret. Thanks Korrey Griffin and Santa Cruz.
Date published: 2016-11-23
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