AKAP 149 Antibody (B-10)recommended for detection of AKAP 149 of mouse, rat and human origin by WB, IP, IF and ELISA

AKAP 149 Antibody (B-10): sc-377450

AKAP 149 Antibody (B-10) is rated 5.0 out of 5 by 1.
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Datasheets

    • AKAP 149 Antibody (B-10) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 604-903 mapping at the C-terminus of AKAP 149 of human origin
    • AKAP 149 Antibody (B-10) is recommended for detection of AKAP 149 of mouse, rat and human origin by WB, IP, IF and ELISA
    • AKAP 149 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of AKAP 149 (B-10): sc-377450.
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What is the recommended starting dilution for Western blot for AKAP 149 (B-10): sc-377450 monoclonal antibody?

Asked by: jerojero
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-377450.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Produced positive Western blot data of AKAP Produced positive Western blot data of AKAP 149 expression in HeLa and LNCaP whole cell lysates. -SCBT QC
Date published: 2014-03-29
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