AKAP 149/121 Antibody (6)recommended for detection of AKAP 149 of human origin and AKAP 121 of mouse and rat origin by WB, IP and IF

AKAP 149/121 Antibody (6): sc-135824

AKAP 149/121 Antibody (6) is rated 4.0 out of 5 by 3.
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Datasheets

    • AKAP 149/121 Antibody (6) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 66-212 of AKAP 149 of human origin
    • recommended for detection of AKAP 149 of human origin and AKAP 121 of mouse and rat origin by WB, IP and IF
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I am using AKAP 149/121 (6): sc-135824 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Professor Griffin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Robust signal in WB in HeLa whole cell lysate Robust signal in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-05-04
Rated 4 out of 5 by from Wonderful immunofluorescence cytoplasmic Wonderful immunofluorescence cytoplasmic staining in A-431 cells. -SCBT QC
Date published: 2015-03-20
Rated 4 out of 5 by from Positive signal in WB in mouse transfected 293T Positive signal in WB in mouse transfected 293T, PC-3 and MCF7 whole cell lysates. -SCBT QC
Date published: 2015-01-23
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