AKAP 12 Antibody (C-12) is a mouse monoclonal IgG1 (kappa light chain) AKAP 12 antibody provided at 200 µg/ml
raised against amino acids 1482-1781 mapping at the C-terminus of AKAP 12 of human origin
AKAP 12 Antibody (C-12) is recommended for detection of AKAP 12 isoforms 1 and 2 of human origin by WB, IP, IF, IHC(P) and ELISA
AKAP 12 Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of AKAP 12 (C-12): sc-376740.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
AKAP 12 Antibody (C-12) is a high quality monoclonal AKAP 12 antibody suitable for the detection of the AKAP 12 antigen. A-kinase anchor protein 12 (AKAP12), also known as Gravin, Ssecks and AKAP250, is a 1,782 amino acid cell growth related protein that is a member of the AKAP family and contains 3 AKAP domains and binds to the dimeric RII-alpha regulatory subunit of PKC. AKAP12 is an anchoring protein that mediates the compartmentalization of protein kinase A (PKA) and protein kinase C (PKC) and serves as a scaffold protein in signal transduction. AKAP12 is expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells with localization in the cell cortex and cytoskeleton, but there does not appear to be expression in platelets, leukocytes, monocytic cell lines or peripheral blood cells. Patients with myasthenia gravis (MG) are able to produce antibodies against the C-terminus of AKAP12. The AKAP12 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat. The human AKAP12 gene maps to chromosome 6q25.1.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rated 3 out of
the diluted product can not be used too many timesAfter receiving the sample, I diluted the product according to the specification. At the first two times exposures, it worked very well. But then it became hard to exposure, the background is very dark. So I think the product is good but maybe can not be used too many times
Date published: 2017-06-14
Rated 5 out of
Produced great immunoperoxidase membraneProduced great immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-04-25
Rated 5 out of
Satisfactory Western Blot data of AKAP 12Satisfactory Western Blot data of AKAP 12 expression in HCT 116 whole cell lysate. -SCBT QC
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