AKAP 12 Antibody (C-12)recommended for detection of AKAP 12 isoforms 1 and 2 of human origin by WB, IP, IF, IHC(P) and ELISA

AKAP 12 Antibody (C-12): sc-376740

AKAP 12 Antibody (C-12) is rated 4.3 out of 5 by 3.
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Datasheets

    • AKAP 12 Antibody (C-12) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1482-1781 mapping at the C-terminus of AKAP 12 of human origin
    • AKAP 12 Antibody (C-12) is recommended for detection of AKAP 12 isoforms 1 and 2 of human origin by WB, IP, IF, IHC(P) and ELISA
    • AKAP 12 Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of AKAP 12 (C-12): sc-376740.
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I am using AKAP 12 (C-12): sc-376740 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from the diluted product can not be used too many times After receiving the sample, I diluted the product according to the specification. At the first two times exposures, it worked very well. But then it became hard to exposure, the background is very dark. So I think the product is good but maybe can not be used too many times
Date published: 2017-06-14
Rated 5 out of 5 by from Produced great immunoperoxidase membrane Produced great immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Satisfactory Western Blot data of AKAP 12 Satisfactory Western Blot data of AKAP 12 expression in HCT 116 whole cell lysate. -SCBT QC
Date published: 2014-07-26
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