AKAP 10 Antibody (A-7)recommended for detection of AKAP 10 of human origin by WB, IP, IF and ELISA

AKAP 10 Antibody (A-7): sc-166220

AKAP 10 Antibody (A-7) is rated 5.0 out of 5 by 3.
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Datasheets

    • AKAP 10 Antibody (A-7) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 508-662 mapping at the C-terminus of AKAP 10 of human origin
    • recommended for detection of AKAP 10 of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended fixation method for immunofluorescence staining with AKAP 10 (A-7): sc-166220 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Superb immunofluorescence cytoplasmic staining Superb immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from Robust signal in WB in human transfected Robust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Good specificity in WB in Caki Good specificity in WB in Caki-1 whole cell lysate. -SCBT QC
Date published: 2015-01-26
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