AIDA-1 Antibody (C-10)recommended for detection of AIDA-1 of human origin by WB, IP, IF and ELISA

AIDA-1 Antibody (C-10): sc-376610

AIDA-1 Antibody (C-10) is rated 5.0 out of 5 by 3.
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Datasheets

    • AIDA-1 Antibody (C-10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 847-1054 mapping near the C-terminus of AIDA-1 of human origin
    • AIDA-1 Antibody (C-10) is recommended for detection of AIDA-1 of human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and canine
    • AIDA-1 Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of AIDA-1 (C-10): sc-376610.
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What is the recommended fixation method for immunofluorescence staining with AIDA-1 (C-10): sc-376610 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from this Ab can be used at 1/500 dilution factor The over-expression of APP in TASTPM mice is clearly demonstrated: I compared this Ab with another Supplier's Ab that is accepted as a "standard" and found very similar immunostaining patterns. In my opinion, this Ab can be used at 1/500 dilution factor, particularly if an overnight incubation is used.
Date published: 2017-02-07
Rated 5 out of 5 by from Great immunofluorescence nuclear and membrane Great immunofluorescence nuclear and membrane staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from Produced positive Western Blot data of AIDA Produced positive Western Blot data of AIDA-1 expression in LNCaP, U-87 MG, NCI-H929 and SK-N-MC whole cell lysates. -SCBT QC
Date published: 2013-03-18
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