AEC, 50X (CAS 132-32-1)

5.0(1)

Alternate Names: 3-amino-9-ethylcarbazole

Application: AEC, 50X is In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product

CAS Number: 132-32-1

Molecular Weight: 210.27

Molecular Formula: C₁₄H₁₄N₂

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

* Refer to Certificate of Analysis for lot specific data (including water content).

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AEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Although not as sensitive as diaminobenzidine (DAB, 50X; sc-24982), the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; sc-24943). Use with AEC Substrate Buffer (sc-36873).

References

1. Graham, R.C., et al. 1965. J. Histochem. Cytochem. 13: 150-152. PMID: 14293042
2. Krenács, T., et al. 1990. Histochem. J. 22: 530-536. PMID: 1705250
3. Okano, K., et al. 1992. Rinsho Byori. 40: 93-97. PMID: 1372054
4. Galey, S., et al. 2003. Orthopedics. 26: 1201-1204. PMID: 14690289

Usage :

• Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps.

• Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X; sc-36873).

• Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well.

• Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT.

• Rinse thoroughly with distilled water. If desired, follow with an appropriate aqueous-based counterstain.

• Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; sc-24943)

• Unused AEC solution can be discarded according to city, county, state, province or country's regulations.

Physical State :

Liquid

Solubility :

Soluble in acetone (50 mg/ml), and DMF. Insoluble in water.

Storage :

Store at 4° C

Refractive Index :

n²⁰_D 1.65

IC50 :

PhoP regulon: EC₅₀50 = 16.14 µM (Salmonella typhimurium); oncogenic and cytokinetic protein MgcRacGAP: IC₅₀ = 64.1 µM (human)

pK Values :

pK_b: 4.99

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

WGK Germany :

RTECS :

FE3590000

PubChem CID :

8588

MDL Number :

MFCD00004964

EC Number :

205-057-7

Beilstein Registry :

174969

SMILES :

CCN1C2=C(C=C(C=C2)N)C3=CC=CC=C31

Download SDS (MSDS)

Certificate of Analysis

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AEC, 50X (CAS 132-32-1) Product Citations

See how others have used AEC, 50X (CAS 132-32-1). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 18 total

PMID: # 18992751 Petersen, KH. et al. 2009. J. Immunol. Methods. 340: 86-89.

PMID: # 19269027 Babister, JC. et al. 2009. Biomaterials. 30: 3174-3182.

PMID: # 17934846 Tobi, M. et al. 2008. Dig. Dis. Sci. 53: 723-729.

PMID: # 18040775 Wu, HY. et al. 2008. Apoptosis. 13: 283-294.

PMID: # 17420353 Jaffer, FA. et al. 2007. Circulation. 115: 2292-2298.

PMID: # 17157828 Sagara, J. et al. 2007. Brain Res. 1131: 11-16.

PMID: # 16780952 Reis, MI. et al. 2007. Mol. Immunol. 44: 774-783.

PMID: # 16210392 Al-Nasiry, S. et al. 2006. Hum. Reprod. 21: 193-201.

PMID: # 16581017 Zhang, XY. et al. 2006. Biochem. Biophys. Res. Commun. 343: 1251-1256.

Citations 1 to 10 of 18 total

Rated 5 out of 5 by SF from Various publications utilize AEC as a substrate Various publications utilize AEC as a substrate for HRP, thus producing a water insoluble red oxidation product. -SCBT Publication Review

Date published: 2015-01-24

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