AEC, 50X CAS: 132-32-1
MF: C14H14N2
MW: 210.27
In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product.

AEC, 50X (CAS 132-32-1)

AEC, 50X | CAS 132-32-1 is rated 5.0 out of 5 by 1.
  • y_2022, m_8, d_19, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_24979, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 115ms
  • REVIEWS, PRODUCT
loading
5
1
4
0
3
0
2
0
1
0
Alternate Names: 3-amino-9-ethylcarbazole
Application: AEC, 50X is In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product
CAS Number: 132-32-1
Molecular Weight: 210.27
Molecular Formula: C14H14N2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
loading

AEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Although not as sensitive as diaminobenzidine (DAB, 50X; sc-24982), the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; sc-24943). Use with AEC Substrate Buffer (sc-36873).


References

1. Graham, R.C., et al. 1965. J. Histochem. Cytochem. 13: 150-152. PMID: 14293042
2. Krenács, T., et al. 1990. Histochem. J. 22: 530-536. PMID: 1705250
3. Okano, K., et al. 1992. Rinsho Byori. 40: 93-97. PMID: 1372054
4. Galey, S., et al. 2003. Orthopedics. 26: 1201-1204. PMID: 14690289

Usage :
• Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps.

• Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X; sc-36873).

• Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well.

• Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT.

• Rinse thoroughly with distilled water. If desired, follow with an appropriate aqueous-based counterstain.

• Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; sc-24943)

• Unused AEC solution can be discarded according to city, county, state, province or country's regulations.
Physical State :
Liquid
Solubility :
Soluble in acetone (50 mg/ml), and DMF. Insoluble in water.
Storage :
Store at 4° C
Refractive Index :
n20D 1.65
IC50 :
PhoP regulon: EC5050 = 16.14 µM (Salmonella typhimurium); oncogenic and cytokinetic protein MgcRacGAP: IC50 = 64.1 µM (human)
pK Values :
pKb: 4.99
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
FE3590000
PubChem CID :
MDL Number :
MFCD00004964
EC Number :
205-057-7
Beilstein Registry :
174969
SMILES :
CCN1C2=C(C=C(C=C2)N)C3=CC=CC=C31

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

AEC, 50X (CAS 132-32-1)  Product Citations

See how others have used AEC, 50X (CAS 132-32-1). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 18 total

PMID: # 30983411  Akbulut, S.|Dogan, Z.|Baskiran, A.|Elbe, H.|Turkoz, Y.| et al. 2019. Biotech Histochem. 1-8.

PMID: # 18992751  Petersen, KH. et al. 2009. J. Immunol. Methods. 340: 86-89.

PMID: # 19269027  Babister, JC. et al. 2009. Biomaterials. 30: 3174-3182.

PMID: # 17934846  Tobi, M. et al. 2008. Dig. Dis. Sci. 53: 723-729.

PMID: # 18040775  Wu, HY. et al. 2008. Apoptosis. 13: 283-294.

PMID: # 17420353  Jaffer, FA. et al. 2007. Circulation. 115: 2292-2298.

PMID: # 17157828  Sagara, J. et al. 2007. Brain Res. 1131: 11-16.

PMID: # 16780952  Reis, MI. et al. 2007. Mol. Immunol. 44: 774-783.

PMID: # 16210392  Al-Nasiry, S. et al. 2006. Hum. Reprod. 21: 193-201.

PMID: # 16581017  Zhang, XY. et al. 2006. Biochem. Biophys. Res. Commun. 343: 1251-1256.

Citations 1 to 10 of 18 total
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 106ms
  • QUESTIONS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Rated 5 out of 5 by from Various publications utilize AEC as a substrate Various publications utilize AEC as a substrate for HRP, thus producing a water insoluble red oxidation product. -SCBT Publication Review
Date published: 2015-01-24
  • y_2022, m_8, d_19, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_24979, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT