AE2 Antibody (D-3)recommended for detection of AE2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

AE2 Antibody (D-3): sc-376632

AE2 Antibody (D-3) is rated 5.0 out of 5 by 2.
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Datasheets

    • AE2 Antibody (D-3) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 100-255 mapping within an N-terminal cytoplasmic domain of AE2 of human origin
    • AE2 Antibody (D-3) is recommended for detection of AE2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • AE2 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of AE2 (D-3): sc-376632.
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The antigen of AE2 (D-3) antibody is cytoplasmic domain. I want to use the antibody for FCM of surface expression of AE2; can you share with me how this antibody works in this type of analysis when the antigen it recognizes is inside not outside the cell?

Asked by: Yingchun Liu
Thank you for your question. Based on the epitope mapping being cytoplasmic for this antibody, we would not recommend using it for detecting surface expression of AE2 by FCM. This is currently the only AE2 antibody we offer in our catalog. Please check back for updates!
Answered by: Technical Support
Date published: 2019-09-11

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-376632: AE2 (D-3) mouse monoclonal antibody will work?

Asked by: Dr Ninau Qelp
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Great Western Blot data of AE2 expression Great Western Blot data of AE2 expression in HeLa and Caki-1 whole cell lysates. -SCBT QC
Date published: 2015-05-11
Rated 5 out of 5 by from Satisfactory Western Blot data of AE2 expression Satisfactory Western Blot data of AE2 expression in HeLa whole cell lysate. -SCBT QC
Date published: 2013-11-11
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