ADAR1 Antibody (15.8.6) recommended for detection of native and recombinant ADAR1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

ADAR1 Antibody (15.8.6): sc-73408

ADAR1 Antibody (15.8.6) is rated 4.8 out of 5 by 16.
  • y_2020, m_10, d_26, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_15
  • loc_en_US, sid_73408, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 192ms
  • REVIEWS, PRODUCT
5
14
4
1
3
1
2
0
1
0

Datasheets

    • ADAR1 Antibody (15.8.6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 440-826 corresponding to the middle region of ADAR1 of human origin
    • Anti-ADAR1 Antibody (15.8.6) is recommended for detection of native and recombinant ADAR1 of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with other members of ADAR family
    • Anti-ADAR1 Antibody (15.8.6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ADAR1 (15.8.6): sc-73408.

Would this antibody detect both the p150 and p110 variants of ADAR1?

Asked by: CrisR
Thank you for your question. The antibody can detect both variants, but what is detected depends on what is present in the samples that are being tested. The expected molecular weight using this antibody is ~136kDa. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2019-05-15

Can the ADAR1 Antibody (15.8.6): sc-73408 mouse monoclonal antibody be used for immunohistochemical staining of mouse tissue?

Asked by: AbPolly
Yes. We recommend using a directly conjugated monoclonal antibody. Please contact our Special Orders department for availability of direct conjugates of ADAR1 Antibody (15.8.6): sc-73408. Otherwise, see ADAR1 (D-8): sc-271854 for ADAR1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647
Answered by: Technical Support 12
Date published: 2017-01-13

What is the difference between sc-sc-73408 and sc-73408 B?

Asked by: AbPolly
Thank you for your inquiry. The product sc-73408 B is our ADAR1 antibody directly conjugated to biotin, great for use in applications such as IHC when doing signal amplification.
Answered by: Technical Service 11
Date published: 2017-01-11

What is the recommended secondary for ADAR1 Antibody (15.8.6): sc-73408 in Western blot?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 5
Date published: 2020-01-01
  • y_2020, m_10, d_25, h_16CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_en_US, sid_73408, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 125ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Detects IFN-inducible bands >100 kD in Western Used in Western with blocking in either milk or BSA. Detects several prominent bands above 100 kDa in human cells lines. Immunoreactivity higher after IFN treatment or virus infection.
Date published: 2020-10-01
Rated 5 out of 5 by from Works well for IF Works well in IF on human fibroblast cells both with MeOH and PFA fixation.
Date published: 2018-07-26
Rated 5 out of 5 by from Works well for endogenous protein Used to detect endogenous protein in fibroblasts of human origin. Worked at dilution of 1:100 and in methanol-fixed cells. Nice subnuclear staining.
Date published: 2018-04-04
Rated 5 out of 5 by from great features Very good western blot result for test mouse brain samples.
Date published: 2018-01-08
Rated 5 out of 5 by from Great Features! A month ago, I used HEK293 to do an antibody test.I found that is excellent ,I did not see other strips beside the different isoforms of adar1.so,I have confidence in santa.
Date published: 2017-07-18
Rated 5 out of 5 by from 这个抗体很赞 ADAR1的抗体很好,特异性很强,我们实验室一直在用,用了大概三年了。未来希望一直可以用,可以一直合作
Date published: 2017-06-14
Rated 3 out of 5 by from 免疫组化做不出 买了两支这样的抗体了,做人源细胞实验没问题,条带清楚,结果好,但是大鼠标本跑western blot条带模糊,做免疫组化完全做不出
Date published: 2017-04-27
Rated 5 out of 5 by from Great for murine samples I requested a sample of this antibody because our Cell signaling one does not recognize murine samples.
Date published: 2017-03-27
  • y_2020, m_10, d_26, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_15
  • loc_en_US, sid_73408, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.