Anti-ADAM32 Antibody (A-5) recommended for detection of ADAM32 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-ADAM32 Antibody (A-5): sc-376738

Anti-ADAM32 Antibody (A-5) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-ADAM32 Antibody (A-5) is a mouse monoclonal IgG1 κ ADAM32 antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 531-680 mapping within an extracellular domain of ADAM32 of human origin
    • ADAM32 Antibody (A-5) is recommended for detection of ADAM32 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-ADAM32 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ADAM32 (A-5): sc-376738.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for ADAM32 Antibody (A-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with ADAM32 Antibody (A-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Can ADAM32 (A-5): sc-376738 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, ADAM32 (A-5): sc-376738 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Strong band in WB in human testis tissue extract Strong band in WB in human testis tissue extract. -SCBT QC
Date published: 2015-03-28
Rated 5 out of 5 by from Robust signal in WB in human transfected Robust signal in WB in human transfected CMV whole cell lysates. -SCBT QC
Date published: 2015-03-08
Rated 5 out of 5 by from Great immunofluorescence membrane staining Great immunofluorescence membrane staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-27
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