Anti-ADAM15 Antibody (D-5) recommended for detection of ADAM15 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-ADAM15 Antibody (D-5): sc-365752

Anti-ADAM15 Antibody (D-5) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-ADAM15 Antibody (D-5) is a mouse monoclonal IgG1 κ ADAM15 antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 561-620 of ADAM15 of human origin
    • Anti-ADAM15 Antibody (D-5) is recommended for detection of ADAM15 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-ADAM15 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ADAM15 (D-5): sc-365752.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ADAM15 Antibody (D-5) for WB applications. These reagents are now offered in bundles with ADAM15 Antibody (D-5) (see ordering information below).
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Hello, would we be able to get this antibody biotinylated?

Asked by: Kathie Day
Thank you for your question. Yes, this antibody is available conjugated to biotin as a special order. Please contact our Special Orders Department to inquire about lead times, and to receive a quote. Please call our Technical Service Department, or email <[email protected]>.
Answered by: Technical Support
Date published: 2019-12-03

What is the recommended fixation method for immunofluorescence staining with ADAM15 (D-5): sc-365752 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Very works with western blotting This antibody very works with western blotting with SAS cells lysate.
Date published: 2020-06-12
Rated 5 out of 5 by from Positive signal in WB in Jurkat and HCT 116 Positive signal in WB in Jurkat and HCT 116 whole cell lysates. -SCBT QC
Date published: 2015-01-22
Rated 5 out of 5 by from Great immunofluorescence cytoplasmic staining Great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-09
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