Acetylethylcholine mustard hydrochloride

Acetylethylcholine mustard hydrochloride (CAS 103994-00-9)

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Synonym: 2-[2-chloroethyl(ethyl)amino]ethyl acetate hydrochloride; Acetyl AF-64
Application: An inhibitor of choactase (choline acetyl-transferase)
CAS Number: 103994-00-9
Purity: ≥97%
Molecular Weight: 230.13
Molecular Formula: C8H16CINO2•HCI
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* Refer to Certificate of Analysis for lot specific data (including water content).
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Acetylethylcholine mustard hydrochloride is an inhibitor of choactase (choline acetyl-transferase). Acetylethylcholine mustard hydrochloride is also a precursor for ethylcholine mustard aziridinium ion (AF-64A). This compound is an irreversible ligand for the high affinity choline transport system. Acetylethylcholine mustard hydrochloride is also a specific presynaptic long action cholinotoxin.


1. Rodríguez, M., et al., Effects of ethylcholine mustard azirinium ion (AF64A) on the choline acetyltransferase and nitric oxide synthase activities in mesopotine cholinergic neurons of the rat. Neuroscience 82, 853-866, (1997) 2. Sandberg, K., et al., AF64A: an active site directed irreversible inhibitor of choline acetyltransferase. J. Neurochem. 44, 439-445, (1985) 3. Moos, W.H., A simplified procedure for preparing the cholinergic neurotoxin ethyl-(2-hydroxyethyl)aziridinium ion (AF64A). Organic Prep. and Proc. Int. 21, 315, (1989)

Appearance :
Physical State :
Solubility :
Soluble in water >5 mg/mL, ethanol, and methanol
Storage :
Desiccate at -20° C
Melting Point :
78-81° C
Density :
1.06 g/cm3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
Transport :
UN 2811, Class 6.1, Packing group II
PubChem CID :
MDL Number :

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Certificate of Analysis

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