Anti-ACE Antibody (5C5) recommended for detection of ACE of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-ACE Antibody (5C5): sc-23909

Anti-ACE Antibody (5C5) is rated 5.0 out of 5 by 2.
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Datasheets

    • ACE Antibody (5C5) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
    • raised against angiotensin-converting enzyme from human lung
    • recommended for detection of ACE of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • available conjugated to agarose for IP
    • See ACE (2E2): sc-23908 for ACE antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ACE Antibody (5C5) for WB and IHC(P) applications. These reagents are now offered in bundles with ACE Antibody (5C5) (see ordering information below).
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Can ACE (5C5): sc-23909 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, ACE (5C5): sc-23909 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Exceptional immunoperoxidase membrane and Exceptional immunoperoxidase membrane and extracellular staining in formalin fixed, paraffin-embedded mouse kidney. -SCBT QC
Date published: 2015-05-12
Rated 5 out of 5 by from Nice pull down in human kidney tissue extracts Nice pull down in human kidney tissue extracts. -SCBT QC
Date published: 2015-04-22
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