Anti-ACE Antibody (2E2) recommended for detection of ACE of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-ACE Antibody (2E2): sc-23908

Anti-ACE Antibody (2E2) is rated 4.7 out of 5 by 17.
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Datasheets

    • Anti-ACE Antibody (2E2) is a mouse monoclonal IgG1 κ ACE antibody, cited in 25 publications, provided at 200 µg/ml
    • raised against angiotensin-converting enzyme from human kidney
    • Anti-ACE Antibody (2E2) is recommended for detection of ACE of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-ACE Antibody (2E2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ACE (2E2): sc-23908.
    • m-IgG1 BP-HRP is the preferred secondary detection reagent for ACE Antibody (2E2) for WB and IHC(P) applications. This reagent is now offered in a bundle with ACE Antibody (2E2) (see ordering information below).
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I used the antibody for IF on fixed (PFA 4%) adherent cells. I tried both RT and O/N 4°C incubation (1:50 dilution) but I didn't work, also changing secondary antibody. Same cells with Flow cytometry (SC23908 PE) resulted positive 90%. What should I do?

Asked by: angelavi
We are sorry to hear that our antibody is not yielding the expected results. Please contact SCBT technical service at [email protected] for help in this matter.
Answered by: Tech Support Europe
Date published: 2021-09-28

What is the recommended secondary reagent to use with ACE Antibody (2E2): sc-23908 for Western blot when working with human samples?

Asked by: Trav11
Thank you for your question. We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-8301 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-24
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Rated 5 out of 5 by from Ottimo funzionamento! The sample worked very well in different cell lines.
Date published: 2021-05-12
Rated 4 out of 5 by from excellent 跑wb哦 不错吧 效果很好 很容易跑, 推荐哦 这个抗体,提高实验效率了 哈哈
Date published: 2019-05-12
Rated 1 out of 5 by from Non funziona per WB Ho provato questo anticorpo in diverse diluizioni e su diverse linee cellulari ma non funziona per Western Blot, nonostante diversi lavori con le versioni precedenti abbiano pubblicato WB funzionanti.
Date published: 2019-02-19
Rated 5 out of 5 by from strong signal Good quality and very cost-effective.It suitable for WB detection of ACE protein, ,We used 1:800 dilution,which can got strong signal.
Date published: 2017-09-02
Rated 5 out of 5 by from good result for ACE I use 6% acrylamide SDS-PAGE gel,and loading 60ug mouse kidney tissue , 100V for 2 hours.This condition may be very proper for this protein.The antibody incubate can be use for 1-2 hour or 4℃ overnight,both good result.
Date published: 2017-07-01
Rated 5 out of 5 by from 抗体效果良好 前一段时间申请的该小样试用装,进免疫荧光鉴定后发现该抗体效果良好,可以做石蜡切片的荧光,基本无杂信号。
Date published: 2017-06-17
Rated 5 out of 5 by from 功能很出色 条带很清晰, 结合力挺强,尽管不在预期的位置,总来说是个不错的抗体.santa的抗体便宜又好用,还会再买.
Date published: 2017-06-09
Rated 5 out of 5 by from good quality western blot and works well at 1:800 dilution in human cell,Great antibody.
Date published: 2017-05-31
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