Ac-VAD-AFC is a fluorogenic caspase-1 substrate. It may also be employed as a caspase-3, caspase-4, or caspase-7 substrate. The reaction can be observed by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
See how others have used Ac-VAD-AFC. Click on the entry to view the PubMed entry .
PMID: # 33748630 Singh, N.|Barnych, B.|Wagner, KM.|Wan, D.|Morisseau, C.|Hammock, BD.| et al. 2021. ACS Omega. 6: 7165-7174.