Ac-DEVD-pNA is a colorimetric substrate for caspase-3 (CPP32) (Km=9.7μM) and related cysteine proteases. It is also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10. The sequence is based on PARP (poly(ADPribose) polymerase) cleavage site Asp216 for caspase-3. Cleavage is monitored colorimetrically at 405nm.
1. Lazebnik, Y.A., et al. 1994. Nature. 371: 346-347. PMID: 8090205 2. Nicholson, D.W., et al. 1995. Nature 376: 37-43. PMID: 7596430 3. Thornberry, N.A. 1994. Methods Enzymol. 244: 615-63. PMID: 7845238 4. Datta, R., et al. 1996. Blood. 88: 1936-1943. PMID: 8822910 5. Talanian, R.V., et al. 1997. J. Biol. Chem. 272: 9677-9682. PMID: 9092497 6. Datta, R., et al. 1997. J. Biol. Chem. 272: 1965-1969. PMID: 8999887
NAc-Asp-Glu-Val-Asp-pNA (pNA = p-Nitroaniline)
Soluble in water (partly), DMSO, buffer, pH 7, and acetonitrile:methanol (1:1) (1 mg/ml).
Store at -20° C
1187.0±65.0° C at 760 mmHg (Predicted)
1.4±0.1 g/cm3 (Predicted)
n20D 1.59 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
This chemical, Ac-DEVD-pNA (CAS 189950-66-1), is sparingly soluble in water. It is recommended to prepare a stock solution in high purity DMSO (>99.9%) and then dilute to an aqueous buffer (pH 7) prior to use.
Rated 5 out of
Various publications cite the use of AcVarious publications cite the use of Ac-DEVD-pNA, a colorimetric substrate, for the detection of caspase-3. It is also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10 with cleavage being monitored colorimetrically at 405nm. -SCBT Publication Review
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