Ac-DEVD-AFC (CAS 201608-14-2)

5.0(1)

Alternate Names: Ac-DEVD-AFC is also known as Caspase-3 Substrate (Fluorogenic).

Application: Ac-DEVD-AFC is a fluorogenic substrate for caspase-3 and related cysteine proteases, used as an indicator for cellular caspase-3 effects.

CAS Number: 201608-14-2

Purity: ≥90%

Molecular Weight: 729.61

Molecular Formula: C₃₀H₃₄F₃N₅O₁₃

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

* Refer to Certificate of Analysis for lot specific data (including water content).

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Ac-DEVD-AFC is a fluorogenic substrate for caspase-3 (CPP32) and related cysteine proteases, which emits a blue to green shift in fluorescence. It also acts as a substrate for granzyme B-processed caspase-6, caspase-7, caspase-8, and caspase-10. It is known that Ac-DEVD-AFC has a greater Stokes′ shift than Ac-DEVD-AMC once it has been cleaved. Since caspase-3 is an effector caspase, it is involved in the cleavage of many substrates which promote apoptosis. This indicator excites light at 400 nm and emits light at 505 nm. In addition, Ac-DEVD-AFC is modeled after Asp216 on caspase-3, the PARP cleavage site. Ac-DEVD-AFC is an essential indicator used to monitor the cellular effects of caspase-3.

References

1. Lazebnik, Y.A., et al. 1994. Nature. 371: 346-347. PMID: 8090205

2. Nicholson, D.W., et al. 1995. Nature 376: 37-43. PMID: 7596430

3. Xiang, J., et al. 1996. Proc. Natl. Acad. Sci. U.S.A. 93: 14559-14563. PMID: 896209

4 .Chandler, J.M., et al. 1998. J. Biol. Chem. 273: 10815-10818. PMID: 9556551

5. Marcelli, M., et al. 1998. Cancer Res. 58: 76-83. PMID: 9426061

Appearance :

Powder

Physical State :

Solid

Sequence :

Ac-Asp-Glu-Val-Asp-AFC

Solubility :

Soluble in 50% acetic acid in water (5 mg/ml), DMF, DMSO, methanol, and slightly soluble water (0.4 mg/ml).

Storage :

Store at -20° C

Boiling Point :

~1152.7° C at 760 mmHg (Predicted)

Density :

~1.5 g/cm³ (Predicted)

Refractive Index :

n²⁰_D 1.57 (Predicted)

Fluoresence :

λex 400 nm, λem 505 nm

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

WGK Germany :

PubChem CID :

644234

MDL Number :

MFCD01310970

SMILES :

CC(C)[[email protected]@H](C(=O)N[[email protected]@H](CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)[[email protected]](CCC(=O)O)NC(=O)[[email protected]](CC(=O)O)NC(=O)C

Download SDS (MSDS)

Certificate of Analysis

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