Anti-ABCG2 Antibody (BXP-53) recommended for detection of ABCG2 of mouse, rat and human origin by WB, IP, IF and FCM

Anti-ABCG2 Antibody (BXP-53): sc-58224

Anti-ABCG2 Antibody (BXP-53) is rated 5.0 out of 5 by 6.
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Datasheets

    • ABCG2 Antibody (BXP-53) is a rat monoclonal IgG2a, cited in 22 publications, provided at 100 µg/ml
    • raised against amino acids 221-394 of ABCG2 of mouse origin
    • recommended for detection of ABCG2 of mouse, rat and human origin by WB, IP, IF and FCM
    • See ABCG2 (B-1): sc-377176 for ABCG2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ABCG2 (BXP-53): sc-58224.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ABCG2 Antibody (BXP-53). This work is in progress.
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Which secondary anti-body will be appropriate for BXP-53? also, does antibodies for mouse-IgG k work as a secondary antibody?

Asked by: Toshi_1993
ABCG2 Antibody (BXP-53) is a rat monoclonal IgG2a. We would recommend using a secondary antibody that is anti-rat IgG2a for detection of this primary antibody. Unfortunately, we do not currently anti-rat IgG secondaries available at this time. Our mouse IgG kappa binding protein will not work as a secondary antibody for detection of rat IgG.
Answered by: Technical Support
Date published: 2018-11-14

I would like to use ABCG2 Antibody (BXP-53): sc-58224 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-58224 is not available directly conjugated, but we have an alternative HNF-3α antibody (sc-377176) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 5
Date published: 2017-02-04
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Rated 5 out of 5 by from Extremely popular ABCG2 antibody The BXP-53 clone is extremely popular choice for detection of ABCG2 by Western blot.
Date published: 2017-01-13
Rated 5 out of 5 by from Reported to be suitable for use in WB using Reported to be suitable for use in WB using mouse spinal cord, cerebral cortex and cerebellum tissue. -SCBT Publication Review
Date published: 2015-04-23
Rated 5 out of 5 by from WB Published with this antibody immunoblot WB Published with this antibody immunoblot analysis using chicken kidney(proximal tubule) tissue. -SCBT Publication Review
Date published: 2015-04-07
Rated 5 out of 5 by from WB positive with human P5 HCC and IGROV1; WB positive with human P5 HCC and IGROV1; Also in canine - MDCKII and MDCK-BCRP cells. -SCBT Publication Review
Date published: 2015-03-12
Rated 5 out of 5 by from Good IF data with human P5 HCC and IGROV1; Good IF data with human P5 HCC and IGROV1; Also in canine - MDCKII and MDCK-BCRP cells. -SCBT Publication Review
Date published: 2015-03-10
Rated 5 out of 5 by from Good signal strength observed in WB using HL Good signal strength observed in WB using HL-60/MX-1, JAR and MCF-7 whole cell lysates. -SCBT QC
Date published: 2013-11-21
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