ABCG2 Antibody (6D171)recommended for detection of ABCG2 of mouse, rat and human origin by WB, IP, IF and IHC(P)

ABCG2 Antibody (6D171): sc-69988

ABCG2 Antibody (6D171) is rated 5.0 out of 5 by 4.
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Datasheets

    • ABCG2 Antibody (6D171) is a mouse monoclonal IgG2a provided at 500 µl supernatant
    • raised against amino acids 271-396 of ABCG2 of human origin
    • recommended for detection of ABCG2 of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with the human MDR1, MRP1 and MRP2 gene products
    • See ABCG2 (B-1): sc-377176 for ABCG2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can sc-69988: ABCG2 (6D171) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, sc-69988: ABCG2 (6D171) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Strong band in WB in HL Strong band in WB in HL-60/MX-1 and JAR whole cell lysates. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Very robust signal in WB in human ABCG2 transfected Very robust signal in WB in human ABCG2 transfected 293T whole cell lysate.
Date published: 2015-04-03
Rated 5 out of 5 by from Positive band in WB in JAR whole cell lysate Positive band in WB in JAR whole cell lysate. -SCBT QC
Date published: 2015-03-09
Rated 5 out of 5 by from Exceptional immunofluorescence membrane staining Exceptional immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-16
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