ABCG2 Antibody (5D3)recommended for detection of ABCG2 of human origin by IP, IF and FCM

ABCG2 Antibody (5D3): sc-18841

ABCG2 Antibody (5D3) is rated 4.7 out of 5 by 7.
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Datasheets

    • ABCG2 Antibody (5D3) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against 3T3 cells transfected with recombinant ABCG2 of human origin
    • ABCG2 Antibody (5D3) is recommended for detection of ABCG2 of human origin by IP, IF and FCM
    • ABCG2 Antibody (5D3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ABCG2 (5D3): sc-18841.
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What is the recommended fixation method for IF with ABCG2 (5D3): sc-18841 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Service 11
Date published: 2017-01-17
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Rated 5 out of 5 by from Great FCM antibody ABCG2 (5D3) works great for IF and FCM. Would not recommend for Western blot (WB).
Date published: 2017-01-11
Rated 5 out of 5 by from We used this antibody in IF in human cancer We used this antibody in IF in human cancer cells and got positive staining.
Date published: 2015-10-23
Rated 4 out of 5 by from IHC; published data on human retinal tumor samples IHC; published data on human retinal tumor samples. -SCBT Publication Review
Date published: 2015-03-14
Rated 5 out of 5 by from Great immunofluorescence membrane staining Great immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-13
Rated 4 out of 5 by from Works well with many different human cell Works well with many different human cell lines: Eca109, Eca109/ABCG2, Eca109/PCDNA3.1 cells. -SCBT Publication Review
Date published: 2015-02-21
Rated 5 out of 5 by from Great antibody for use in IHC Great antibody for use in IHC, publishable data obtained using human brain tissue samples. -SCBT Publication Review
Date published: 2015-01-07
Rated 5 out of 5 by from Superb FCM data of HL Superb FCM data of HL-60/MX1 cells. -SCBT QC
Date published: 2014-06-01
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