Anti-ABCA4 Antibody (3F4) recommended for detection of ABCA4 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-ABCA4 Antibody (3F4): sc-65672

Anti-ABCA4 Antibody (3F4) is rated 3.8 out of 5 by 4.
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Datasheets

    • ABCA4 Antibody (3F4) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
    • raised against the 220-kDa ABCA4 protein of bovine origin
    • Anti-ABCA4 Antibody (3F4) is recommended for detection of ABCA4 of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine
    • ABCA4 Antibody (3F4) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ABCA4 (3F4): sc-65672.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ABCA4 Antibody (3F4) for WB and IHC(P) applications. These reagents are now offered in bundles with ABCA4 Antibody (3F4) (see ordering information below).
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Will this anti-ABCA4 (SC-65672) bind ABCA4 ECD1 or ECD2? Need for Flow cytometry... Thx

Asked by: Anonymous
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined. We do not know whether or not the antibody binds to ECD1 or ECD2.
Answered by: Tech Service 1
Date published: 2020-12-10

I purchased your ABCA4 (3F4) sc-65672 antibody last year and tried it on fibroblast cells following one of the published paper but I didn't get the expected band. Can you help me with that? Thanks.

Asked by: Di Huang
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-09-11

Hello I wish to know if it is possible to recieve the 3F4 ABCA4 antibody conjugated to agarose or sepharose. And if so how the 3F4-immunoaffinity column should be prepared.

Asked by: Tess555
Thank you for your question. We provide sc-65672 conjugated to agarose for IP experiments. If you require more information, please contact our Technical Service https://www.scbt.com/scbt/resources/technical-service
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with ABCA4 Antibody (3F4): sc-65672?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 3 out of 5 by from Not so good as other ABC family antibody The band is not clear enough, there are many inspecialized bands on the film.
Date published: 2018-01-30
Rated 4 out of 5 by from Nice western Very clean western blot data; would like to see staining
Date published: 2017-01-10
Rated 5 out of 5 by from Reliable and Versatile Antibody Strong, clear signal in WB with little to no background. Very Reliable. Works well in a variety of species including mouse, rat, bovine, and human samples,
Date published: 2016-12-22
Rated 3 out of 5 by from Light but positive band in WB in mouse eye Light but positive band in WB in mouse eye tissue extract. -SCBT QC
Date published: 2014-01-09
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