8-Anilino-1-naphthalenesulfonic acid ammonium saltA fluorescent probe for protein studies

8-Anilino-1-naphthalenesulfonic acid ammonium salt (CAS 28836-03-5)

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Synonym: 1,8-ANS NH4; ANSA; Ammonium 8-anilino-1-naphthalenesulfonate
Application: A fluorescent probe for protein studies
CAS Number: 28836-03-5
Purity: 95%
Molecular Weight: 316.37
Molecular Formula: C16H13NO3S•NH3
* Refer to Certificate of Analysis for lot specific data (including water content).
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8-Anilino-1-naphthalenesulfonic acid is a fluorescent dye that binds with high affinity to hydrophobic surfaces of proteins. It is commonly used to study molecular assemblies of surfactants and amphiphilic polymers because a blue shift of the emission maximum indicates the fluorophore is located in less polar media.


References

R.A. Muesing, T. Nishida, Disruption of low- and high-density human plasma lipoproteins and phospholipid dispersions by 1-anilinonaphthalene-8-sulfonate. Biochemistry 10, 2952, (1971); U.P. Andley, B. Chakrabarti Biochemistry 20, 1687, (1981); J.J. Ewbank, T.E. Creighton Biochemistry 32, 3694, (1993); J.A. Vinson, J.E. Hooyman, For the sensitive detection of neutral lipids and phospholipids after TLC. J. Chromatogr. 135, 226, (1977)

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water, 1N NaOH, and methanol.
Storage :
Store at room temperature
Melting Point :
242-244° C
Boiling Point :
582.9° C at 760 mmHg (Predicted)
IC50 :
Nuclear receptor subfamily 4 group A member 1: EC5050 = 2.95 µM (human)
Fluoresence :
λex 388 nm, λem 470 nm in 0.1M Tris,0.2 M KCl, PH 9.0, BSA
pK Values :
pKa: 0.19 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
120066
MDL Number :
MFCD00012560
EC Number :
249-265-6
Beilstein Registry :
3581235
SMILES :
C1=CC=C(C=C1)NC2=CC=CC3=C2C(=CC=C3)S(=O)(=O)[O-].[NH4+]

Download SDS (MSDS)

Certificate of Analysis

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