5prime-DeoxythymidineA cell synchronizing nucleoside

5′-Deoxythymidine (CAS 3458-14-8)

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Synonym: 2′,5′-Dideoxythymidine
Application: A cell synchronizing nucleoside
CAS Number: 3458-14-8
Purity: 98%
Molecular Weight: 226.23
Molecular Formula: C10H14N2O4
* Refer to Certificate of Analysis for lot specific data (including water content).
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5'-Deoxythymidine is a research tool for antiviral and anticancer studies. 5'-Deoxythymidine has been employed in studying the entry mechanism into human erythrocytes in comparison with other deoxythymidines. Deoxythymidine is the DNA nucleoside T, which pairs with deoxyadenosine (A) in double-stranded DNA. In cell biology it is employed to synchronize the cells in S phase. As part of one of the 4 nucleotides in DNA, it is a naturally occurring compound that exists in all living organisms and DNA viruses. RNA has uridine (uracil joined to ribose) instead. Uracil is chemically very similar to thymine, the latter being 5-methyluracil. Since thymine nucleotides are precursors of DNA, and not RNA, the prefix "deoxy" is often left out, i.e., deoxythymidine is often just called thymidine.


1. Ishii, M., et al., 1989. Enzymatic production of 5-methyluridine from purine nucleosides and thymine by Erwinia carotovora AJ-2992. Agric. Biol. Chem., 53 (12), 3209-18.

Appearance :
Crystalline powder
Physical State :
Solubility :
Soluble in water.
Storage :
Store at room temperature
Melting Point :
192-193° C (lit.)
Boiling Point :
486.56° C (Predicted)
Density :
~1.3 g/cm3 (Predicted)
Refractive Index :
n20D 1.56 (Predicted)
Optical Activity :
α28/D 8.9°, c = 0.6 in water
pK Values :
pKa: 9.55 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
PubChem CID :
MDL Number :

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Certificate of Analysis

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