5-(Iodoacetamido)fluoresceinA thiol reactive fluorescein derivative

5-(Iodoacetamido)fluorescein (CAS 63368-54-7)

5-(Iodoacetamido)fluorescein | CAS 63368-54-7 is rated 5.0 out of 5 by 1.
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Synonym: 5-IAF
Application: A thiol reactive fluorescein derivative
CAS Number: 63368-54-7
Molecular Weight: 515.25
Molecular Formula: C22H14INO6
* Refer to Certificate of Analysis for lot specific data (including water content).
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5-(Iodoacetamido)fluorescein is a thiol reactive fluorescein derivative for synthesis of fluorescently labeled organelles, proteins and peptides at or near their physiological pH value (pH 7) including enzymes (e.g. (Na, K)-ATPase) or organelles like nuclei and nuclear matrices.


References

W. Ip, M.E. Fellows, Fluorescent thiol reagent. Fluorescent measurement of desmin intermediate filament assembly. Anal. Biochem. 185, 10, (1990); R.F. Murphy, Label in flow cytofluorometric analysis. J. Biol. Chem. 257, 1695, (1982)

Appearance :
powder
Physical State :
Solid
Storage :
Store at -20° C
Melting Point :
265-267° C
Fluoresence :
λex 493 nm, λem 522 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
123822
SMILES :
C1=CC2=C(C=C1NC(=O)CI)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O

Download SDS (MSDS)

Certificate of Analysis

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Rated 5 out of 5 by from Various publications cite the use of 5 Various publications cite the use of 5-(Iodoacetamido)fluorescein, a thiol reactive fluorescein derivative, for synthesis of fluorescently labeled organelles, proteins and peptides at or near their physiological pH value (pH 7) including enzymes (e.g. (Na, K)-ATPase) or organelles like nuclei and nuclear matrices. -SCBT Publication Review
Date published: 2015-02-22
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