5-Carboxy-X-rhodamine N-succinimidyl ester CAS: 344402-35-3
MF: C37H33N3O7
MW: 631.67
A nucleotide labeling reagent.

5-Carboxy-X-rhodamine N-succinimidyl ester (CAS 344402-35-3)

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Alternate Names: 5-ROX,SE
Application: 5-Carboxy-X-rhodamine N-succinimidyl ester is a nucleotide labeling reagent
CAS Number: 344402-35-3
Molecular Weight: 631.67
Molecular Formula: C37H33N3O7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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5-Carboxy-X-rhodamine N-succinimidyl ester is a labeling reagent used in the preparation of charge-modified dye-labeled Dideoxynucleotide Triphosphates (ddNTP) to "direct-load" DNA sequencing.


References

1. Nucleic Acids Res. 20, 2471, (1992) 2. Anal. Biochem. 223, 39, (1994) 3. P.J. Finn et al. Nucleic Acids Res. 30, 2877, (2002)

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in DMF and Acetonitrile
Storage :
Store at -20° C
Fluoresence :
λex 575 nm, λem 600 nm in 0.1 M phosphate pH 7.0
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
SMILES :
C1CC2=C3C(=C4C(=C2)C(=C5C=C6CCC[N+]7=C6C(=C5O4)CCC7)C8=C(C=C(C=C8)C(=O)ON9C(=O)CCC9=O)C(=O)[O-])CCCN3C1

Download SDS (MSDS)

Certificate of Analysis

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