5-Bromo-2′-deoxyuridineA thymidine analog useful for DNA labelling

5-Bromo-2′-deoxyuridine (CAS 59-14-3)

5-Bromo-2′-deoxyuridine | CAS 59-14-3 is rated 5.0 out of 5 by 2.
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Synonym: 5-Bromouracil deoxyriboside; 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil
Application: A thymidine analog useful for DNA labelling
CAS Number: 59-14-3
Purity: ≥98%
Molecular Weight: 307.10
Molecular Formula: C9H11BrN2O5
* Refer to Certificate of Analysis for lot specific data (including water content).
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5-Bromo-2'-deoxyuridine is a thymidine analog. Useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. 5-Bromo-2'-deoxyuridine can be used to label DNA. The resulting DNA can be used in conjunction with BrdU Monoclonal Antibody for the immunohistochemical analysis of cell proliferation.


References

Garabedian, E.M., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 15382. Cattoretti, G., et al. 1993. J. Pathol. 171, 83. Fukuda, K., et al. 1990. Anal. Quant. Cytol. Histol. 12, 135.

Appearance :
Crystalline powder
Physical State :
Solid
Solubility :
Soluble in water (10 mg/mL), NH4OH (0.1 M) at 20° C, DMF, DMSO, and sodium hydroxide.
Storage :
Store at -20° C
Melting Point :
191-194° C (lit.)(dec.)
Density :
~1.91 g/cm3
Refractive Index :
n20D 1.65 (Predicted)
Optical Activity :
α25/D +22.9°, c = 1% in water; α20/D +31°, c = 1 in 0.1N HCl
IC50 :
L5178Y : IC50 = 12 µM (Mus musculus)
Ki Data :
Thymidylate kinase: Ki= 5 µM (Mycobacterium tuberculosis)
pK Values :
pKa: 7.85
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
2
RTECS :
YU7350000
PubChem CID :
6035
Merck Index :
14: 1452
MDL Number :
MFCD00006529
EC Number :
200-415-9
Beilstein Registry :
30395
SMILES :
C1[C@@H]([C@H](O[C@H]1N2C=C(C(=O)NC2=O)Br)CO)O

Download SDS (MSDS)

Certificate of Analysis

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What is the recommended dose of inoculation?

Asked by: DavRoq
Thank you for your question. The researchers of the following publication cite 0.5 mg/ml BDU. http://www.jbc.org/content/264/22/13043.full.pdf
Answered by: Technical Support
Date published: 2017-07-20

Podria indicarme con que referencias anticuerpos es compatible este producto: 5-Bromo-2'-deoxyuridinesc-290815 Gracias

Asked by: Cassio
Thank you very much for your question. 5-Bromo-2'-deoxyuridine can be used to label DNA. The resulting DNA can be used in conjunction with BrdU Monoclonal Antibody for the immunohistochemical analysis of cell proliferation. We currently offer different antibodies for the detection of BrdU, e.g. our most popular antibody is BrdU Antibody (IIB5) sc-32323. However, for the detection of BrdU incorporated into DNA during S-phase of a cell cycle by IF, IHC(P) and FCM we recommend the BrdU Antibody (MoBU-1): sc-51514.
Answered by: Technical Support Europe
Date published: 2017-07-14

What is the appearance of the compound?

Asked by: two2igm05
Thank you for your question. This chemical, 5-Bromo-2'-deoxyuridine: sc-290815, has the appearance of a white solid.
Answered by: Chemical Support 2
Date published: 2017-07-13
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Rated 5 out of 5 by from Works great! We have been using this BrdU for more than a year. It has been working perfectly without any problem.
Date published: 2019-04-29
Rated 5 out of 5 by from Numerous publications cite the use of 5 Numerous publications cite the use of 5-Bromo-2'-deoxyuridine, a thymidine analog, to label DNA, typically in cell proliferation studies. -SCBT Publication Review
Date published: 2015-06-26
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