Date published: 2025-10-19

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5(6)-Carboxyfluorescein diacetate (CAS 124387-19-5)

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Alternate Names:
5(6)-CFDA
Application:
5(6)-Carboxyfluorescein diacetate is a derivitized fluorescein compound
CAS Number:
124387-19-5
Purity:
≥90%
Molecular Weight:
460.39
Molecular Formula:
C25H16O9
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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5(6)-Carboxyfluorescein diacetate is a cell-permeant, non-fluorescent substrate that is used to label live cells for various experimental assays. Once inside the cell, esterases cleave the acetate groups, resulting in the formation of 5(6)-carboxyfluorescein, which is a highly fluorescent compound. This allows for the visualization and tracking of live cells in a variety of experimental applications, such as cell migration, proliferation, and viability assays. The mechanism of action involves the esterase-mediated cleavage of the acetate groups, leading to the release of the fluorescent compound within the cell. This enables researchers to monitor and analyze cellular processes and behaviors in real-time, providing insights into cell biology and function. 5(6)-carboxyfluorescein diacetate can combination with other fluorescent dyes or markers to study complex cellular interactions and dynamics. 5(6)-Carboxyfluorescein diacetate serves for investigating various cellular processes.


5(6)-Carboxyfluorescein diacetate (CAS 124387-19-5) References

  1. 5,6-carboxyfluorescein diacetate succinimidyl ester-labeled apoptotic and necrotic as well as detergent-treated cells can be traced in composite cell samples.  |  Dumitriu, IE., et al. 2001. Anal Biochem. 299: 247-52. PMID: 11730350
  2. Selection of bacteriophage-resistant mutants of Streptococcus thermophilus.  |  Viscardi, M., et al. 2003. J Microbiol Methods. 55: 109-19. PMID: 14500002
  3. Intracellular pH and metabolic activity of long-chain dicarylic acid-producing yeast Candida tropicalis.  |  Liu, S., et al. 2003. J Biosci Bioeng. 96: 349-53. PMID: 16233535
  4. A xylem sap retrieval pathway in rice leaf blades: evidence of a role for endocytosis?  |  Botha, CE., et al. 2008. J Exp Bot. 59: 2945-54. PMID: 18617505
  5. 5(6)-Carboxyfluorescein diacetate as an indicator of Caenorhabditis elegans viability for the development of an in vitro anthelmintic drug assay.  |  Gnoula, C., et al. 2007. Talanta. 71: 1886-92. PMID: 19071538
  6. HBx transfection limits proliferative capacity of podocytes through cell cycle regulation.  |  Zhang, Y., et al. 2014. Acta Biochim Biophys Sin (Shanghai). 46: 1016-23. PMID: 25395163
  7. Shootward Movement of CFDA Tracer Loaded in the Bottom Sink Tissues of Arabidopsis.  |  Jiang, M., et al. 2019. J Vis Exp.. PMID: 31132061
  8. LncRNA KCNQ1OT1 delayed fracture healing through the Wnt/β-catenin pathway.  |  Gu, H., et al. 2019. Eur Rev Med Pharmacol Sci. 23: 4575-4583. PMID: 31210283
  9. Symplasmic phloem unloading and post-phloem transport during bamboo internode elongation.  |  Deng, L., et al. 2020. Tree Physiol. 40: 391-412. PMID: 31976532
  10. Selective protein photocleavage by fluorescein derivatives.  |  Jityuti, B., et al. 2020. J Photochem Photobiol B. 212: 112027. PMID: 32977112
  11. Staphylococcus aureus membrane-damaging activities of four phenolics.  |  Bouarab, L., et al. 2021. FEMS Microbiol Lett. 368: PMID: 34173656
  12. Detection of Glycolytically Active Lacticaseibacillus paracasei Strain Shirota by Flow Cytometry Targeting the Efflux Activity of Fluorescent Dye: a Potential Tool for Quality Assessment of Probiotic Cells in Milk Products.  |  Kubota, H., et al. 2023. Appl Environ Microbiol. 89: e0215622. PMID: 37022200
  13. Direct Infusion Device for Molecule Delivery in Plants.  |  Rhodes, BH., et al. 2023. J Vis Exp.. PMID: 37335099
  14. Selective labeling of embryonic neurons cultured on astrocyte monolayers with 5(6)-carboxyfluorescein diacetate (CFDA).  |  Petroski, RE. and Geller, HM. 1994. J Neurosci Methods. 52: 23-32. PMID: 8090014
  15. Carboxyfluorescein diacetate labeling does not affect adhesion molecule expression or function in human neutrophils or eosinophils.  |  Davenpeck, KL., et al. 1995. J Immunol Methods. 188: 79-89. PMID: 8551041

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

5(6)-Carboxyfluorescein diacetate, 25 mg

sc-210423
25 mg
$56.00