4E-BP1 Antibody (P-1)recommended for detection of 4E-BP1 of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P) and ELISA

4E-BP1 Antibody (P-1): sc-9977

4E-BP1 Antibody (P-1) is rated 4.5 out of 5 by 33.
  • y_2019, m_12, d_13, h_13
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_33
  • loc_en_US, sid_9977, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 53ms
  • REVIEWS, PRODUCT
5
26
4
3
3
0
2
1
1
3

Datasheets

    • 4E-BP1 Antibody (P-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full length 4E-BP1 of human origin
    • 4E-BP1 Antibody (P-1) is recommended for detection of 4E-BP1 of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P) and ELISA
    • 4E-BP1 Antibody (P-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of 4E-BP1 (P-1): sc-9977.
Submit a review for this product and receive 15 CruzCredits
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 81ms
  • QUESTIONS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Rated 1 out of 5 by from doesn't work It did not work in dilutions 1:200, 1: 500. I wasted my money
Date published: 2019-08-09
Rated 1 out of 5 by from Used to be a great antibody and now... I have used this anti-total 4EBP1 antibody before and had very good results. I'm using PVDFand the Licor TBS Odyssey blocking buffer and Odyssey detection, with the primary at 1:200. I am getting almost no signal at all with the most recent batch of Alexa labelled sc-9977 (which was ordered in May 2019 because 3 other vials I had in the 'frig had stopped working). I have 14 blots with mouse tissue lysates which gave beautiful signals with 6 other antibodies, but not sc-9977. This is very frustrating!! I don't want to have to strip 14 blots to reprobe with a CST antibody but it looks like it may be my only choice. In the attached photo, there is a nice band for actin at the correct size and another for S6 at the right size butnothing between 14kD and 20kD (the size of total 4EBP1). No other probes used should show the band at a little less than 25kD- and the total 4EBP1 antibody isn't reacting with the phospho 4EBP1 picked up nicely by a CST antibody. I wonder if the sc-9977 antibody is picking up something else??
Date published: 2019-06-14
Rated 5 out of 5 by from 抗体不错 为了配套检测mTORC1这条信号通路,我申请了这个蛋白的两种抗体,磷酸化修饰的有杂带,但是总蛋白还是很好的,条带清晰而单一。
Date published: 2017-11-07
Rated 5 out of 5 by from Quite clear band for wb of rat protein 1:500 diluted for wb of rat protein ,the band is strong and special.
Date published: 2017-10-20
Rated 5 out of 5 by from grateful it works very well. I can't express my gratitude for the antibody.
Date published: 2017-10-05
Rated 5 out of 5 by from 关于对4-E-BP1抗体功能的评价 Great antibody for western blotting, can be detected in HEK293T, HCT116 and DLD1 whole cell lysates with strong and clear band.
Date published: 2017-09-22
Rated 5 out of 5 by from Great features! I got free sample several months ago, and I used the recommanded dilution rate ,and the western blot was nice, but I didn't use it in immunohistochemical! Thank you!
Date published: 2017-09-04
Rated 5 out of 5 by from reliable quality This 4E-BP1 antibody works very well for western (1:800),it give strong signal for human pancreas tissue detection.The quality is very reliable.
Date published: 2017-08-12
  • y_2019, m_12, d_13, h_13
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_33
  • loc_en_US, sid_9977, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 3ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.