4E-BP1 Antibody (D-10)recommended for detection of 4E-BP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

4E-BP1 Antibody (D-10): sc-514073

4E-BP1 Antibody (D-10) is rated 5.0 out of 5 by 3.
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Datasheets

    • 4E-BP1 Antibody (D-10) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-25 at the N-terminus of 4E-BP1 of human origin
    • recommended for detection of 4E-BP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • blocking peptide, sc-514073 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See 4E-BP1 (P-1): sc-9977 for 4E-BP1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What application is the blocking peptide sc-514073 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Western blot works great at 1:500 dilution Western blot works great at 1:500 dilution, giving a very specific signal at the right size of 4EBP1.
Date published: 2015-10-05
Rated 5 out of 5 by from Good Western Blot data of 4E Good Western Blot data of 4E-BP1 expression in non-transfected and human 4E-BP1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from Good Western Blot data of 4E Good Western Blot data of 4E-BP1 expression in HeLa nuclear extract and K-562, A-431, HL-60 and Hep G2 whole cell lysates. -SCBT QC
Date published: 2013-12-28
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