4-Nitrophenyl-N-acetyl-beta- D-glucosaminideA substrate for rapid colorimetric assays in human urine

4-Nitrophenyl-N-acetyl-β- D-glucosaminide (CAS 3459-18-5)

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Synonym: p-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside PNP-β-D-GlcNAc
Application: A substrate for rapid colorimetric assays in human urine
CAS Number: 3459-18-5
Purity: ≥99%
Molecular Weight: 342.30
Molecular Formula: C14H18N2O8
* Refer to Certificate of Analysis for lot specific data (including water content).
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4-Nitrophenyl-N-acetyl-β- D-glucosaminide is a useful substrate for rapid colorimetric assay of N-acetyl-b-glucosaminidase activity in human urine. Chromogenic β-Glucosaminidase substrate yielding a yellow solution upon cleavage, employed particularly in yeasts and molds.


References

1. Steigerwald, J C., et al., 1973. The assessment of lysosomal glycosidases in normal skin. Biochimica et biophysica acta. 321(1): 256-61. PMID: 4750767
2. Jones, C S., et al., 1980. Purification, properties, kinetics, and mechanism of beta-N-acetylglucosamidase from Aspergillus niger. The Journal of biological chemistry. 255(24): 11861-9. PMID: 7440573

Appearance :
Crystalline powder
Physical State :
Solid
Solubility :
Soluble in water (~5 g/l), DMSO, and ethanol.
Storage :
Store at -20° C
Melting Point :
210-212° C
Boiling Point :
694.74° C at 760 mmHg (Predicted)
Density :
1.51 g/cm3 (Predicted)
Refractive Index :
n20D 1.62 (Predicted)
Optical Activity :
α20/D -15°, c = 0.5 in water
pK Values :
pKa: 12.76 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
102416
MDL Number :
MFCD00063696
EC Number :
222-398-7
Beilstein Registry :
96193
SMILES :
CC(=O)N[C@@H]1[C@H]([C@@H]([C@H](O[C@H]1OC2=CC=C(C=C2)[N+](=O)[O-])CO)O)O

Download SDS (MSDS)

Certificate of Analysis

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