4-Methylumbelliferyl β-D-N,N',N"-triacetylchitotrioside is a fluorogenic substrate that has been used for the detection of lysozyme, peptidoglycan, muramidase, and endochitinase activity. Cleavage of this substrate produces a fluorescent product, 4-methylumbelliferone (λex 360 nm, λem 455 nm).
1. Yang, Y. and Hamaguchi, K. 1980. J. Biochem. 87: 1003-1014. PMID: 7390976 2. Hollis, T., et al. 1997. Arch. Biochem. Biophys. 344: 335-342. PMID: 9264547 3. Howard, M.B., et al. 2003. J. Bacteriol. 185: 3352-3360. PMID: 12754233 4. Telkov, M.V., et al. 2006. Biochemistry Mosc. 71: 414-422. PMID: 16615861
Soluble in DMSO (10-20 mg/ml), methanol: water (1: 1) (10 mg/ml), and pyridine: water (1: 1) (4.90-5.10 mg/ml).
Store at -20° C
~1211.61° C at 760 mmHg (Predicted)
~1.6 g/cm3 (Predicted)
λex 360 nm, λem 455 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Thank you for your question.
This product is soluble in DMSO (10-20 mg/ml), methanol: water (1: 1) (10 mg/ml), and pyridine: water (1: 1) (4.90-5.10 mg/ml).
Please contact Technical Service if you have further questions concerning this product.
Rated 5 out of
ChangChang, et al. (PubMed ID 25437446) used 4-Methylumbelliferyl -D-N,N',N''-triacetylchitotrioside as a substrate in the process of purifying acidic chitinase from black soybean seeds (via ammonium sulfate fractionation and 3 successive steps of column chromatography). -SCBT Publication Review
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