4-Aminophenyl b-D-glucuronide Sodium Salt (CAS 21080-66-0)

4-Aminophenyl β-D-glucuronide, a conjugated molecule, serves as a pivotal substance in biochemical assays and analytical chemistry research, particularly in the study of enzyme activities and metabolic pathways. Its structure comprises a phenyl ring attached to an amine group (4-aminophenyl), which is further linked through a glycosidic bond to a β-D-glucuronic acid moiety. This unique configuration makes it an ideal substrate for β-glucuronidase, an enzyme that catalyzes the hydrolysis of β-D-glucuronic acid residues from the non-reducing end of glycosaminoglycans. The enzymatic activity towards 4-Aminophenyl β-D-glucuronide is exploited in biochemical assays to quantitatively measure β-glucuronidase activity, which is for understanding various biological processes, including the detoxification pathways in cells and the metabolism of xenobiotics. Additionally, its cleavage by β-glucuronidase releases 4-aminophenol, a compound that can be easily detected and quantified, further underscoring its utility in facilitating the exploration of enzyme kinetics and the elucidation of metabolic mechanisms at the molecular level.