3611-RF + PMA nuclear extract: sc-2144

The 3611-RF + PMA nuclear extract is sourced from the 3611-RF cell line, a type of fibroblast, which has been treated with phorbol 12-myristate 13-acetate (PMA), a compound known to activate protein kinase C (PKC) and modulate various signaling pathways within cells. This nuclear extract contains concentrated nuclear proteins, including transcription factors, co-regulators, and other nuclear-associated molecules, providing a snapshot of the cellular nuclear environment under the influence of PMA. The treatment with PMA typically induces changes in gene expression, cell differentiation, and proliferation, making this extract highly valuable for studying the nuclear responses to PKC activation. Researchers utilize the 3611-RF + PMA nuclear extract in various experimental contexts to investigate the mechanisms by which external stimuli like PMA impact nuclear functions, particularly focusing on transcriptional regulation and chromatin remodeling. This extract is instrumental in exploring how signaling pathways communicate with the nuclear machinery, affecting the transcriptional profiles and potentially leading to modifications in cellular behavior. By employing this nuclear extract in studies, scientists can gain deeper insights into the dynamic nature of gene regulation and the complex molecular interactions within the nucleus that drive cellular responses to external signals. Such research contributes broadly to our understanding of cell biology and the regulation of gene expression.