3-Carboxyumbelliferyl b-D-galactopyranoside is a fluorogenic substrate. It releases free 3-carboxyumbelliferone upon bet-galactosidase enzyme activity. 3-caboxyumbelliferone has the following fluorescent activity: λex = 386, λem 445 nm, ε = 32K. In acidic conditions (below pKa 7.8) the absorption shifts to lower wavelengths (235-340 nm) and fluorescence intensity decreases.
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