Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well. Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8. Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.
1. Laemmli, U.K. 1970. Nature. 227: 680-685. PMID: 5432063
2. Wu, M., et al. 2009. Diabetes Metab. Res. Rev. 25: 279-286. PMID: 19177471
3. Wu, M., et al. 2009. PLoS ONE. 4: e6430. PMID: 19641606
See how others have used 2X Laemmli Sample Buffer. Click on the entry to view the PubMed entry .
PMID: # 27100161 Choh, V. et al. 2016. Investigative ophthalmology & visual science. 57: 2140-51.
PMID: # 26983346 Zhao, GX. et al. 2016. Biotech Histochem. 91: 269-76.
PMID: # 24629563 Yang, SJ. et al. 2014. Metab. Clin. Exp. 63: 693-701.
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