2prime,7prime-Dichlorodihydrofluorescein DiacetateA cell-permeable fluorogenic ROS probe

2′,7′-Dichlorodihydrofluorescein Diacetate (CAS 4091-99-0)

2′,7′-Dichlorodihydrofluorescein Diacetate, CAS 4091-99-0 is rated 5.0 out of 5 by 1.
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Synonym: DCFH-DA; DCFH; 2',7'-Dichlorofluorescin diacetate
Application: A cell-permeable fluorogenic ROS probe
CAS Number: 4091-99-0
Molecular Weight: 487.29
Molecular Formula: C24H16Cl2O7
* Refer to Certificate of Analysis for lot specific data (including water content).
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2′,7′-Dichlorofluorescin Diacetate is a cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.

Usage :
For maximum solubility in aqueous buffers, DCFH-DA can be directly disolved in 0.1 M Na2CO3 (5 mg/ml) and then immediately diluted with PBS (pH 7.2) to achieve the desired concentration or pH. Upon neutralization of the solution the concentration may not be greater than 1 mg/ml. We do not recommend storing the aqueous solution for more than one day.
Physical State :
Solid
Solubility :
Soluble in DMSO (25 mg/ml), 100% ethanol (25 mg/ml), DMF (25 mg/ml), and aqueous buffers (sparingly soluble).
Storage :
Store at -20° C
Melting Point :
232° C
Boiling Point :
~593.5° C at 760 mmHg (Predicted)
Density :
~1.5 g/cm3 (Predicted)
Refractive Index :
n20D 1.64 (Predicted)
Fluoresence :
λex 502 nm, λem 523 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
77718
MDL Number :
MFCD00128955
Beilstein Registry :
4610273
SMILES :
CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl

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Certificate of Analysis

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2prime,7prime-Dichlorodihydrofluorescein Diacetate  Product Citations

See how others have used 2prime,7prime-Dichlorodihydrofluorescein Diacetate. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 13 total

PMID: # 31143124  Trachtenberg, A.|Muduli, S.|Sidoryk, K.|Cybulski, M.|Danilenko, M.| et al. 2019. Front Pharmacol. 10: 507.

PMID: # 31038133  Zhao, Y.|Zhou, Y.|Wang, M.| et al. 2019. Food Funct.

PMID: # 30352681  Jeong, DH.|Choi, YN.|Seo, TW.|Lee, JS.|Yoo, SJ.| et al. 2018. Biochem. Biophys. Res. Commun. 506: 423-428.

PMID: # 28672983  Zhao, J. et al. 2017. Exp Ther Med. 14: 664-670.

PMID: # 29050275  Zhao, Y.|Li, ETS.|Wang, M.| et al. 2017. Oncotarget. 8: 70239-70249.

PMID: # 28565770  Ma, XW. et al. 2017. Exp Ther Med. 13: 1799-1805.

PMID: # 26811107  Pandurangan, M. et al. 2016. Biol Trace Elem Res. 173: 62-70.

PMID: # 27000946  Pandurangan, M. et al. 2016. J. Mol. Recognit. 29: 426-35.

PMID: # 26276565  Pandurangan, M. et al. 2016. Biol Trace Elem Res. 170: 309-19.

PMID: # 26985734  Pandurangan, M. et al. 2016. J. Photochem. Photobiol. B, Biol. 158: 206-11.

Citations 1 to 10 of 13 total
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Rated 5 out of 5 by from Various publications cite the use of 2 Various publications cite the use of 2 ,7 -Dichlorofluorescin Diacetate, a cell-permeable fluorogenic probe, for the detection of reactive oxygen species (ROS) and nitric oxide ( NO) and for the determination of the degree of overall oxidative stress. -SCBT Publication Review
Date published: 2015-07-05
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