2prime,7prime-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein CAS: 85138-49-4
MF: C27H20O11
MW: 520.44
Fluorescent cytoplasmic pH indicator.

2′,7′-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (CAS 85138-49-4)

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Alternate Names: BCECF
Application: 2′,7′-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein is Fluorescent cytoplasmic pH indicator
CAS Number: 85138-49-4
Purity: ≥90%
Molecular Weight: 520.44
Molecular Formula: C27H20O11
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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2',7'-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (BCECF) is a widely used fluorescent indicator for the measurement of cytoplasmic pH in cells. BCECF has been used to: observe regulation of basal acid secretion by glycogen synthase kinase 3, identify enzymatic activity, determine transport activity in human erythrocytes, observe Na+/H+ exchange activity and regulation of gastric acid release.


References

1. Rink, T.J., et al. 1982. J. Cell Biol. 95: 189-196. PMID: 6815204
2. Rotte, A., et al. 2010. Biochem. Biophys. Res. Commun. 398: 677-682. PMID: 20609358
3. Rotte, A., et al. 2010. Cell. Physiol. Biochem. 25: 695-704. PMID: 20511715
4. Wesołowska, O., et al. 2010. Eur. J. Pharmacol. 644: 32-40. PMID: 20633549
5. Rotte, A., et al. 2010. J Gastroenterol. 45: 1022-1032. PMID: 20552232
6. Foladori, P., et al. 2010. Water Res. 44: 3807-3818. PMID: 20537673

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (slightly), DMSO, DMF, and methanol.
Storage :
Store at -20° C
Boiling Point :
936.23° C at 760 mmHg (Predicted)
Density :
1.68 g/cm3 (Predicted)
Refractive Index :
n20D 1.74 (Predicted)
Fluoresence :
λex 503 ± 3 nm, λem 527 ± 4 nm in Buffer pH 9; λex 482 nm, λem 520 nm in 0.1 M Tris pH 7.0
pK Values :
pKa: 6.97
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
MDL Number :
MFCD00036970
Beilstein Registry :
8375770
SMILES :
C1=CC(=C(C=C1C(=O)O)C2=C3C=C(C(=O)C=C3OC4=CC(=C(C=C42)CCC(=O)O)O)CCC(=O)O)C(=O)O

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Certificate of Analysis

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2prime,7prime-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (CAS 85138-49-4)  Product Citations

See how others have used 2prime,7prime-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (CAS 85138-49-4). Click on the entry to view the PubMed entry .

Citations 1 to 3 of 3 total

PMID: # 21545783  Di Bernardo, J. et al. 2011. Anal. Biochem. 415: 32-38.

PMID: # 11749926  Effros, RM. et al. 2001. Am. J. Med. 111 Suppl 8A: 56S-59S.

PMID: # 11600438  Galietta, LV. et al. 2001. Am. J. Physiol., Cell Physiol. 281: C1734-C1742.

Citations 1 to 3 of 3 total
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