20α-Hydroxy Cholesterol is an allosteric activator of the Hedgehog signaling pathway Smoothened (Smo) oncoprotein that binds at a site distinct from the canonical cyclopamine binding site. 20α-Hydroxy Cholesterol activates Hedgehog (Hh) signaling (EC50 ~ 3μM for induction of Hh reporter gene transcription in in NIH 3T3 cells). It induces Smo accumulation in primary cilia and also exhibits osteogenic activity and activates the liver X receptor (LXR).
1. Higley, N A., et al., 1984. The steatotic and cytotoxic effects of cholesterol oxides in cultured L cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 22(12): 983-92. PMID: 6542547
2. Kilsdonk, E P., et al., 1995. Inhibition of cellular cholesterol efflux by 25-hydroxycholesterol. Journal of lipid research. 36(3): 505-16. PMID: 7775862
3. Cantwell, H., et al., 1998. The response of the antioxidant defense system in rat hepatocytes challenged with oxysterols is modified by Covi-ox. Cell biology and toxicology. 14(6): 401-9. PMID: 9879932
Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.