2,2,7-trimethylguanosine Antibody (K121) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against trimethylguanosine-keyhole limpet hemocyanin conjugates
2,2,7-trimethylguanosine Antibody (K121) is recommended for detection of 5` terminal 2,2,7-trimethylguanosine of all major snRNAs of mouse, rat and human origin by IP, IF and IHC(P)
2,2,7-trimethylguanosine Antibody (K121) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of 2,2,7-trimethylguanosine (K121): sc-32724.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Pre-mRNA splicing is a critical step in the post-transcriptional regulation of gene expression. Several protein complexes are involved in proper mRNA splicing and transport. The small nuclear ribonucleoprotein particles (snRNPs) interact with the SRm160/300 splicing coactivator complex to form a large RNA spliceosome. 2,2,7-trimethylguanosine is a nucleoside at the 5' terminal cap site of the snRNA (small ribonucleoprotein RNA) componenet of U1, U2, U4/U6 and U5 nuclear snRNPs. U-type snRNPs mediate intron splicing of precursor mRNA. Antibodies recognizing the 5' terminal cap motif can inhibit mRNA splicing. Anti-trimethylguanosine (TMG) monoclonal, K121, can immunoprecipitate all major nuclear snRNPs.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Thank you for your question. We provide 200 ug in 1 ml.
Answered by: Technical Support
Date published: 2019-07-13
What secondary should be used with this mouse monoclonal primary antibody?
Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
Create a new account
Email address already exists, please enter a new valid email address.Hide
USE YOUR SOCIAL NETWORK
Create an account quickly and easily with your preferred social network account. You won't have to remember an extra name and password.
Creating an account with us makes your shopping experience much easier and faster. You can save favorites, save cart, check order status and speed through checkout with saved addresses, payment methods and more.