13S-Hydroxy-9Z,11E-octadecadiene-(2-biotinyl)hydrazide

13S-Hydroxy-9Z,11E-octadecadiene-(2-biotinyl)hydrazide, a compound derived from octadecadienoic acid, has gained prominence in research due to its biotinylated moiety, which confers avidin-biotin binding properties. This chemical serves as a valuable tool in various biochemical and biotechnological applications. One notable application is its use as a probe in affinity chromatography and pull-down assays to study protein-protein interactions. By conjugating the biotinylated hydrazide to proteins of interest, researchers can isolate and identify protein complexes or study protein-ligand interactions with high specificity and affinity. Additionally, this compound has been employed in enzyme assays, where the biotin tag enables facile detection and quantification of enzyme activity or substrate binding. Moreover, its hydrophobic octadecadiene backbone facilitates membrane permeability, making it suitable for labeling and tracking biomolecules within cellular environments. Furthermore, the biotinylated hydrazide′s stability and specificity make it a preferred choice for applications requiring sensitive detection and precise targeting, such as fluorescence imaging and biosensor development. Overall, 13S-Hydroxy-9Z,11E-octadecadiene-(2-biotinyl)hydrazide holds promise as a versatile tool in biochemical research, offering insights into complex biological processes and facilitating the development of innovative research methodologies.