10-Pyrene-PC is a substrate for all PLA2s with the exception of cPLA2 and PAF-AH. Upon phospholipid hydrolysis of 10-pyrene-PC, this compound is produced. The monomeric 10-pyrenyldecanoic acid exhibits fluorescence (excitation 345 nm, emission 395 nm), allowing quantitation of phospholipase activity.
1 Roberts, M.F. 1996. FASEB J. 10: 1159-1172. PMID: 8751718 2 Lusa, S., et al. 1996. Biochem J. 315: 947-952. PMID: 8645181
A solution in chloroform
Store at -20° C
λex 341 nm, λem 375 nm in methanol
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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