1,2-bis(Heptanoylthio)glycerophosphocholine is a synthetic thiol phosphoglyceride lipid derived from D-mannitol which functions as a substrate for PLA2 (phospholipase A2). Hydrolysis of 1,2-bis(Heptanoylthio)glycerophosphocholine by PLA2 produces free thiols, which may be derivitized with dithionitrobenzoic acid to form the chromophore for a spectrophotometric assay of intracellular and membrane-bound PLA2 activity. Release of Arachidonic Acid (sc-200770) from membrane phospholipids mediated by PLA2 is thought to regulate eicosanoid production and marked PLA2 activity is observed in correlation with inflammation states. Inhibitors of PLA2 activity, presenting potential intervention upon the inflammatory role of PLA2, have been identified using this assay design. 1,2-bis(Heptanoylthio)glycerophosphocholine is a substrate of PLA1.
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