USP9X/Y 항체 (E-12) WB, IP, IF, IHC(P)와 ELISA으로 USP9X of mouse, rat and human orgin, and USP9Y of human origin 을 검출할것을 권장합니다.

USP9X/Y 항체 (E-12): sc-365353

USP9X/Y 항체 (E-12) is rated 4.8 out of 5 by 11.
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데이터 시트

    • 안티-USP9X/Y 항체 E-12 는 마우스 monoclonal IgG2a κ USP9X/Y 항체, 5간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 USP9X/Y을 항원으로 사용하였습니다.
    • USP9X/Y 항체(E-12)는 WB, IP, IF, IHC(P) and ELISA으로 USP9X of mouse, rat and human orgin, and USP9Y of human origin 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-USP9X/Y 항체 (E-12)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • USP9X/Y (E-12): sc-365353무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for USP9X/Y Antibody (E-12) for WB and IHC(P) applications. These reagents are now offered in bundles with USP9X/Y Antibody (E-12) (see ordering information below).
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Can USP9X/Y (E-12): sc-365353 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, USP9X/Y (E-12): sc-365353 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from WB Tested in SHSY-5Y human neuroblastoma cells (~15-20ug/lane), dilution 1:1000 in 3% milk in TBST. Got a single clear band at expected size after very long exposure.
Date published: 2018-05-15
Rated 5 out of 5 by from 功能很出色 抗体使用不错,申请的小样包装,抗体稀释比例是一比五百,WB能做几次,抗体特异性还可以,条带清晰,抗体不错
Date published: 2017-09-11
Rated 5 out of 5 by from helpful I used it on WB, the cell is SH-SY5Y, the results are good!
Date published: 2017-08-14
Rated 4 out of 5 by from Good Endogeous USP9X was detected in 293 cell lysates with a strong intensity.
Date published: 2017-08-08
Rated 5 out of 5 by from Good I am able to detect endogenous USP9X in 293 cell lysates.
Date published: 2017-08-04
Rated 5 out of 5 by from good Ubiquitin controls many intracellular processes, such as signal transduction, transcriptional activation, and cell cycle progression, through the use of a broad range of enzymes that can be added or removed. Thank you for providing a well-behaved product
Date published: 2017-02-02
Rated 5 out of 5 by from Great USP9X/Y monoclonal choice for WB Strong clean bands for WB in NIH/3T3, HeLa and MCF7 whole cell lysates.
Date published: 2017-01-18
Rated 5 out of 5 by from mouse against USP9x/y antibody I used this antibody for WB, IP and IF. It works very well.
Date published: 2016-12-15
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