USP34 항체 (3H9) WB, IP, IF, IHC(P) 와ELISA으로 human origin의 USP34을 검출할것을 권장합니다.

USP34 항체 (3H9): sc-100631

USP34 항체 (3H9) is rated 4.8 out of 5 by 4.
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데이터 시트

    • 안티-USP34 항체 3H9 는 마우스 monoclonal IgG2a κ USP34 항체, 1간행물에 인용, 이며 100 µg/ml으로 제공합니다.
    • human origin의 USP34의 전체인 3296-3396 아미노산을 항원으로 사용하였습니다.
    • WB, IP, IF, IHC(P) 와ELISA으로 human origin의 USP34을 검출할것을 권장합니다.
    • USP34 (3H9): sc-100631무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for USP34 Antibody (3H9) for WB and IHC(P) applications. These reagents are now offered in bundles with USP34 Antibody (3H9) (see ordering information below).
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What percentage gel/ recommended transfer conditions do you suggest for a good band for USP34? I have used recommended dilution of 1:200 and see multiple bands (of similar intensity) above 250kd MW marker band.

Asked by: SMahajan
Thank you for your question. This is a very large protein, so we recommend a 8% gel for this protein. Please contact our Technical Service Department if you need further assistance with this antibody.
Answered by: Tech Service
Date published: 2019-02-28

What peptide has been used as the immunogen for this antibody?

Asked by: lms104
Thank you for your question. USP34 (3H9): sc-100631 was raised against amino acids 2396-3396 of human recombinant USP34 (accession# Q70CQ2). Please contact our technical service directly for additional information.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Can sc-100631: USP34 (3H9) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, sc-100631: USP34 (3H9) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Perfect! I have used it for western blot and IP in HEK 293T cell extract and it perfectly works.
Date published: 2020-10-20
Rated 5 out of 5 by from Produced beautiful immunoperoxidase nuclear Produced beautiful immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human seminoma tissue. -SCBT QC
Date published: 2015-04-17
Rated 5 out of 5 by from Positive band seen in WB in A549 whole cell lysate Positive band seen in WB in A549 whole cell lysate. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by from Strong band detected in WB in HeLa whole Strong band detected in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-27
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