USP25 항체 (C-5) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 USP25을 검출할것을 권장합니다.

USP25 항체 (C-5): sc-398414

USP25 항체 (C-5) is rated 5.0 out of 5 by 4.
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데이터 시트

    • 안티-USP25 항체 C-5 는 마우스 monoclonal IgG1 κ USP25 항체, 3간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 USP25에서 C-terminus에 위치한 851-990 아미노산을 항원으로 사용하였습니다.
    • 안티-USP25 항체 (C-5)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 USP25 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-USP25 항체 (C-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • USP25 (C-5): sc-398414무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for USP25 Antibody (C-5) for WB applications. These reagents are now offered in bundles with USP25 Antibody (C-5) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with USP25 (C-5): sc-398414 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 非常好用,WB实验结果显示无杂带。 我一个半月前订个该抗体试用装,使用效果不错,无杂带,相对较容易显影。只是试用装量少,只够做一次WB。
Date published: 2017-08-15
Rated 5 out of 5 by from Excellent antibody! The USP25 Antibody (C-5): sc-398414 provides good staining pattern for IF, showing cytoplasmic localization.
Date published: 2017-01-19
Rated 5 out of 5 by from Excellent immunofluorescence cytoplasmic Excellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-05
Rated 5 out of 5 by from Great Western Blot data of USP25 expression Great Western Blot data of USP25 expression in NTERA-2 cl.D1 and MIA PaCa-2 whole cell lysates. -SCBT QC
Date published: 2014-03-04
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