USP18 항체 (E-5) WB, IP, IF 와ELISA으로 human origin의 USP18을 검출할것을 권장합니다.

USP18 항체 (E-5): sc-374064

USP18 항체 (E-5) is rated 2.3 out of 5 by 3.
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데이터 시트

    • 안티-USP18 항체 E-5 는 마우스 monoclonal IgG1 κ USP18 항체, 2간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 USP18을 항원으로 사용하였습니다.
    • USP18 항체 (E-5)는 WB, IP, IF and ELISA으로 human유래의 USP18 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-USP18 항체 (E-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • USP18 (E-5): sc-374064무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for USP18 Antibody (E-5) for WB applications. These reagents are now offered in bundles with USP18 Antibody (E-5) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-374064: USP18 (E-5) monoclonal antibody?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 1 out of 5 by from 検出できません。 インターフェロン刺激後のヒト細胞ライセートを用いたましたが、内在性USP18を検出できませんでした。
Date published: 2020-10-16
Rated 1 out of 5 by from Did not work. No bands were observed when probing untreated and interferon treated human fibroblast whole cell lysates. Bands were observed when using a different USP18 primary antibody.
Date published: 2016-12-19
Rated 5 out of 5 by from Produced nice Western blot data of USP18 Produced nice Western blot data of USP18 expression in non-transfected and human USP18 transfected CMV whole cell lysates. -SCBT QC
Date published: 2014-01-31
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