ULBP1 항체 (3F1) IP, IF 와FCM으로 human origin의 ULBP1을 검출할것을 권장합니다.

ULBP1 항체 (3F1): sc-53131

ULBP1 항체 (3F1) is rated 3.8 out of 5 by 6.
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데이터 시트

    • 안티-ULBP1 항체 3F1 는 마우스 monoclonal IgM κ ULBP1 항체, 5간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against purified human ULBP1
    • IP, IF 와FCM으로 human origin의 ULBP1을 검출할것을 권장합니다.
    • also provided azide-free for blocking, sc-53131 L, 200 µg/0.1 ml
    • ULBP1 (3F1): sc-53131무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ULBP1 Antibody (3F1). This work is in progress.
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Which secondaries are good for immunofluorescence? Do I need IgM secondaries?

Asked by: Kale
Thank you for your question. This mouse monoclonal antibody, ULBP1 (3F1): sc-53131, is an IgM and using an isotype-specific secondary antibody (anti-mouse IgM) is recommended. Alternatively, we also recommend using our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to a fluorochrome, such as fluorescein (FITC), m-IgGκ BP-FITC: sc-516140. Other fluorochromes available include: m-IgGκ BP-CFL 488: sc-516176, m-IgGκ BP-CFL 555: sc-516177, or m-IgGκ BP-CFL 594: sc-516178. We have tested and confirmed reactivity of our mouse IgG kappa binding protein with mouse IgM.
Answered by: Technical Support
Date published: 2017-11-22

I bought the L form of this antibody, sc-53131 L for blocking (neutralization). How long can I store this at in the refridgerator?

Asked by: Trav11
The L form of the antibody should be stored at -20°C. Once thawed, we recommend using as soon as possible, although it is usually stable for up to 2 weeks at 4 degrees.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from Very faint signal by FCM This antibody was tested by indirect FCM on HFF cells but does not give a very clean signal, even at dilution 1:50.
Date published: 2018-11-02
Rated 4 out of 5 by from Very good antibody I got the sample from santa cruz last month. This work perfectly in the lung fibroblasts that we work with. I am really glad that it worked as I did not find any other better antibody. Good luck...
Date published: 2018-01-17
Rated 5 out of 5 by from Good Fluorescent Staining This ULBP1 antibody shows good cytoplasmic staining when used with methanol-fixed HeLa cells in IF. Also works in Flow Cytometry.
Date published: 2017-02-02
Rated 4 out of 5 by from IHC(P) publishable data with human HCC tissues IHC(P) publishable data with human HCC tissues - SCBT Publication Review
Date published: 2015-05-02
Rated 4 out of 5 by from Clear immunofluorescence cytoplasmic staining Clear immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-08
Rated 4 out of 5 by from Good indirect FCM data of C1R mock and C1R Good indirect FCM data of C1R mock and C1R-ULBP1 transfectants stained with ULBP1 -SCBT QC
Date published: 2014-09-29
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