TIMP-2 항체 (3A4) WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 TIMP-2을 검출할것을 권장합니다.

TIMP-2 항체 (3A4): sc-21735

TIMP-2 항체 (3A4) is rated 5.0 out of 5 by 13.
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데이터 시트

    • 안티-TIMP-2 항체 3A4 는 마우스 monoclonal IgG2a κ TIMP-2 항체, 63간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 TIMP-2에서 내부에 위치한 56-66 아미노산을 항원으로 사용하였습니다.
    • 안티-TIMP-2 항체 (3A4)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 TIMP-2 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-TIMP-2 항체 (3A4)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • TIMP-2 (3A4): sc-21735무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for TIMP-2 Antibody (3A4) for WB and IHC(P) applications. These reagents are now offered in bundles with TIMP-2 Antibody (3A4) (see ordering information below).
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How can TIMP-2 (3A4): sc-21735 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody, c-Myc (9E10), is currently available conjugated to phycoerythrin (sc-21735 PE), fluorescein (sc-21735 FITC), Alexa Fluor® 488 (sc-21735 AF488) or Alexa Fluor® 647 (sc-21735 AF647)
Answered by: Technical Support 13
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TIMP-2 (3A4): sc-21735?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-12
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Rated 5 out of 5 by from Clear Staining for IF Showed clear staining with concentrations of 1:50 and 1:100 on human primary cells
Date published: 2018-08-21
Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from works as advertised Antibody well for western blot of tissue homogenates
Date published: 2018-03-29
Rated 5 out of 5 by from A very good product I used this antibody in Western blot for my mouse brain samples. A single band was detected in all protein lysates. It is a great product.
Date published: 2017-11-06
Rated 5 out of 5 by from outstanding product We had been struggling to detect TIMP-2 in our human cell line using other company's product for a long time,however, some clear bands have been shown in our lab by using this product(sc-21735).
Date published: 2017-07-03
Rated 5 out of 5 by from Excellent Antibody I tried for Western blot in brain cell lysates. It gave strong band. Good antibody.
Date published: 2017-05-30
Rated 5 out of 5 by from Outstanding product Great data for all applications including WB,IF and IHC
Date published: 2017-01-30
Rated 5 out of 5 by from Good TIMP-2 detection Antibody produces a strong signal in IF and IHC(P) for TIMP-2. Also bands cleanly in WB. Great for mouse, rat, and human samples.
Date published: 2017-01-27
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