STEAP 항체 (B-4) WB, IP, IF 와ELISA으로 human origin의 STEAP을 검출할것을 권장합니다.

STEAP 항체 (B-4): sc-271872

STEAP 항체 (B-4) is rated 5.0 out of 5 by 2.
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데이터 시트

    • 안티-STEAP 항체 B-4 는 마우스 monoclonal IgG1 κ STEAP 항체, 6간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 STEAP에서 내부에 위치한 1-105 아미노산을 항원으로 사용하였습니다.
    • STEAP 항체 (B-4)는 WB, IP, IF and ELISA으로 human유래의 STEAP 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-STEAP 항체 (B-4)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • STEAP (B-4): sc-271872무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for STEAP Antibody (B-4) for WB applications. These reagents are now offered in bundles with STEAP Antibody (B-4) (see ordering information below).
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Is this antibody(sc-271872;STEAP antibody) suitable for immunostaining and what is the recommended starting dilution? If not, is there another STEAP1 antibody suitable for IF?

Asked by: April123
Yes this is approved for use in immunostaining applications. We recommend a starting dilution of 1:50. For more information, please refer to our immunohistochemistry protocol.
Answered by: Dallas Tech Service
Date published: 2021-04-07

Is this antibody(sc-271872;STEAP antibody) suitable for immunostaining? If so, what is the recommended starting dilution?

Asked by: Anonymous
Thank you for your question. This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates! We recommend a starting dilution of 1:50 (range 1:50-1:500).
Answered by: Technical Support
Date published: 2020-09-13

What is the recommended starting dilution for Western blot for sc-271872: STEAP (B-4) monoclonal antibody?

Asked by: Professor Griffin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Nice antibody for western blot We used this antibody for expression detection of STEAP by western blot. We were able to get nice bands around the correct molecular weight
Date published: 2017-02-03
Rated 5 out of 5 by from Produced nice Western blot data of STEAP Produced nice Western blot data of STEAP expression in LNCaP whole cell lysate. -SCBT QC
Date published: 2013-07-01
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